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GalT-RpHLuorin2 vector (V017538)

Basic Vector Information

Vector Name:
GalT-RpHLuorin2
Antibiotic Resistance:
Kanamycin
Length:
4959 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Selection Marker:
Neo/G418
Promoter:
CMV
5' Primer:
CMV-F
Growth Strain(s):
DH5a
Growth Temperature:
37℃

GalT-RpHLuorin2 vector Map

Copy Sequence View Map
GalT-RpHLuorin24959 bp6001200180024003000360042004800CMV enhancerCMV promoterratiometric pHluorinSV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRHSV TK poly(A) signalori

GalT-RpHLuorin2 vector Sequence

LOCUS       .                       4959 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        108..411
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        412..615
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             958..1668
                     /label="ratiometric pHluorin"
                     /note="pH-sensitive mutant of green fluorescent protein
                     (Miesenböck et al., 1998)"
     polyA_signal    1794..1915
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1922..2377)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2404..2508
                     /label="AmpR promoter"
     promoter        2510..2867
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2902..3693
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    3928..3975
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4304..4892
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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