Basic Vector Information
- Vector Name:
- PBT3-SUC-Spike(SARS-Cov-2)
- Antibiotic Resistance:
- Kanamycin
- Length:
- 11443 bp
- Type:
- Hybridization
- Replication origin:
- ori
- Host:
- Yeast
- Selection Marker:
- LEU2
- 5' Primer:
- T3
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
PBT3-SUC-Spike(SARS-Cov-2) vector Map
PBT3-SUC-Spike(SARS-Cov-2) vector Sequence
LOCUS . 11443 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 26..44
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
CDS 358..4176
/gene="S"
/label="S"
/note="Spike glycoprotein from Severe acute respiratory
syndrome coronavirus 2. Accession#: P0DTC2"
CDS 4483..5088
/label="LexA"
/note="E. coli DNA-binding protein that represses genes
involved in the SOS response to DNA damage"
CDS 5107..5340
/label="VP16 AD"
/note="transcriptional activation domain of herpes simplex
virus protein VP16 (Triezenberg et al., 1988; Cousens et
al., 1989)"
terminator 5345..5591
/label="CYC1 terminator"
/note="transcription terminator for CYC1"
promoter complement(5610..5628)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin 5795..6250
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6550..6954
/label="LEU2 promoter"
CDS 6967..8058
/label="LEU2"
/note="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
CDS complement(8782..9594)
/label="KanR"
/note="aminoglycoside phosphotransferase"
rep_origin 10036..10624
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(10801..11302)
/label="CEN/ARS"
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
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