Basic Vector Information
- Vector Name:
- pK19pA-TurboID
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4068 bp
- Type:
- Protein-Protein Interaction
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- 5' Primer:
- lac P
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pK19pA-TurboID vector Map
pK19pA-TurboID vector Sequence
LOCUS . 4068 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
protein_bind 42..63
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 78..108
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 116..132
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
primer_bind 140..156
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 179..196
/label="6xHis"
/note="6xHis affinity tag"
CDS 197..622
/label="protein A"
/note="immunoglobulin binding domains of Staphylocuccus
aureus protein A"
CDS 641..1597
/label="TurboID"
/note="mutant of the E. coli biotin ligase BioID engineered
for improved catalytic efficiency (Branon et al., 2018)"
primer_bind complement(1635..1651)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
CDS 2156..2947
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
rep_origin 3234..3822
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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