pSL1521 (pSPIN, pSC101* backbone) vector (V017531)

LOCUS       .                      12724 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        241..332
                     /label="AmpR promoter"
     CDS             333..1121
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        1310..1344
                     /label="J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119)"
     CDS             8326..8337
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     terminator      9786..9833
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             9988..10644
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     rep_origin      11470..11692
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             11740..12687
                     /label="Rep101(Ts)"
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"