Basic Vector Information
- Vector Name:
- pDL278_P23-DsRed-Express2
- Antibiotic Resistance:
- Spectinomycin
- Length:
- 7462 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Gram-positive bacteria, Enterococcus faecalis
- Copy Number:
- Low
- 5' Primer:
- lac-P
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 30℃
pDL278_P23-DsRed-Express2 vector Map
pDL278_P23-DsRed-Express2 vector Sequence
LOCUS . 7462 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 1..31
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 39..55
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
primer_bind 63..79
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 244..918
/label="DsRed-Express2"
/note="noncytotoxic tetrameric variant of DsRed fluorescent
protein (Strack et al., 2008)"
primer_bind complement(989..1005)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
terminator 1479..1565
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1657..1684
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(1980..2023)
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin 6401..6989
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 7427..7448
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
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