At2S3:RUBY vector (V017529)

LOCUS       .                      14085 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    complement(178..202)
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             652..2142
                     /gene="CYP76AD1"
                     /label="CYP76AD1"
                     /note="Cytochrome P450 76AD1 from Beta vulgaris.
                     Accession#: I3PFJ5"
     CDS             2152..2208
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     terminator      4610..4858
                     /label="HSP terminator"
                     /note="efficient transcription terminator from the
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya
                     et al., 2010)"
     promoter        5338..5521
                     /label="NOS promoter"
                     /note="nopaline synthase promoter"
     CDS             5573..6583
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     terminator      6636..6888
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(7701..7725)
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8246..9034
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      9283..9871
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(10057..10197)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10541..10735)
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10804..11874)
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(12306..12932)
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"