Basic Vector Information
- Vector Name:
- pRPS5A-PTP-3Xflag-rbc L 294 Left_G1397 N
- Antibiotic Resistance:
- Spectinomycin
- Length:
- 12842 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Plants
- Selection Marker:
- Basta
- 5' Primer:
- Forward(left ITR)
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pRPS5A-PTP-3Xflag-rbc L 294 Left_G1397 N vector Map
pRPS5A-PTP-3Xflag-rbc L 294 Left_G1397 N vector Sequence
LOCUS . 12842 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
protein_bind complement(5..25)
/label="attB4"
/note="core recombination site for the Gateway® BP
reaction"
CDS 1857..1922
/label="3xFLAG"
/note="three tandem FLAG® epitope tags, followed by an
enterokinase cleavage site"
misc_feature 5029..5053
/label="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
CDS 6384..7010
/label="pVS1 StaA"
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 7442..8512
/label="pVS1 RepA"
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 8581..8775
/label="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 9119..9259
/label="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(9445..10033)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10282..11070)
/label="SmR"
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
misc_feature 11591..11615
/label="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
terminator complement(11708..11960)
/label="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(11983..12531)
/label="BlpR"
/note="phosphinothricin acetyltransferase"
promoter complement(12573..12752)
/label="NOS promoter"
/note="nopaline synthase promoter"
This page is informational only.