Basic Vector Information
- Vector Name:
- pCDF-GFPplus
- Antibiotic Resistance:
- Streptomycin
- Length:
- 4442 bp
- Type:
- Protein expression
- Replication origin:
- CloDF13 ori
- Host:
- Bacteria
- 5' Primer:
- T7
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pCDF-GFPplus vector Map
pCDF-GFPplus vector Sequence
LOCUS . 4442 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
CDS 86..799
/label="GFPuv"
/note="GFP variant optimized for excitation by UV light"
terminator 872..966
/label="lambda t0 terminator"
/note="transcription terminator from phage lambda"
terminator 1054..1101
/label="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(1275..2063)
/label="SmR"
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
promoter complement(2064..2155)
/label="AmpR promoter"
rep_origin complement(2203..2941)
/label="CloDF13 ori"
/note="Plasmids containing the CloDF13 (CDF) origin of
replication can be propagated in E. coli cells that contain
additional plasmids with compatible origins."
protein_bind complement(3117..3138)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(3154..4233)
/label="lacI"
/note="lac repressor"
promoter complement(4234..4311)
/label="lacI promoter"
promoter 4357..4375
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 4376..4400
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
RBS 4415..4437
/label="RBS"
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
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