pLac-NifA-Tac-signal-TPI-signal-Hyg-Tet vector (V017523)

LOCUS       .                       7240 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        196..224
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             272..1459
                     /label="TcR"
                     /note="tetracycline efflux protein"
     promoter        complement(1637..1655)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1660..1676)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1751..2773)
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     promoter        complement(2843..2947)
                     /label="AmpR promoter"
     rep_origin      3526..4114
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        4314..4344
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4352..4368
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     4376..4392
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             4412..5983
                     /gene="nifA"
                     /label="nifA"
                     /note="Nif-specific regulatory protein from Klebsiella
                     pneumoniae. Accession#: P03027"
     promoter        6028..6056
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     terminator      complement(7007..7034)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(7126..7212)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"