Basic Vector Information
- Vector Name:
- pEX18Tc-FlhA(PAO1)
- Antibiotic Resistance:
- Tetracycline
- Length:
- 8457 bp
- Type:
- Gene-editing vector
- Replication origin:
- ori
- Host:
- E. coli
- Selection Marker:
- SacB
- Copy Number:
- Low
- 5' Primer:
- lac P
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pEX18Tc-FlhA(PAO1) vector Map
pEX18Tc-FlhA(PAO1) vector Sequence
LOCUS . 8457 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 96..200
/label="AmpR promoter"
CDS 275..1462
/label="TcR"
/note="tetracycline efflux protein"
rep_origin 1739..2327
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2615..2636
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2651..2681
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 2689..2705
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
primer_bind 2713..2729
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4907..4923)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
terminator 5273..5359
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 5451..5478
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
oriT 5899..6008
/label="oriT"
/note="incP origin of transfer"
promoter 6382..6827
/label="sacB promoter"
/note="sacB promoter and control region"
CDS 6828..8246
/label="SacB"
/note="secreted levansucrase that renders bacterial growth
sensitive to sucrose"
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