Cart 2

pGAPZAdaro22AE vector (V017520)

Basic Vector Information

Vector Name:
pGAPZAdaro22AE
Antibiotic Resistance:
Zeocin
Length:
4308 bp
Type:
Protein expression
Replication origin:
ori
Host:
Yeast
Selection Marker:
Bleo
Promoter:
GAP
5' Primer:
GAP-P
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pGAPZAdaro22AE vector Map

Copy Sequence View Map
pGAPZAdaro22AE4308 bp600120018002400300036004200GAP promoterAOX1 terminatorTEF1 promoterEM7 promoterBleoRCYC1 terminatorori

pGAPZAdaro22AE vector Sequence

LOCUS       .                       4308 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        7..483
                     /label="GAP promoter"
                     /note="promoter for Pichia pastoris
                     glyceraldehyde-3-phosphate dehydrogenase"
     terminator      2129..2375
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     promoter        2390..2801
                     /label="TEF1 promoter"
                     /note="promoter for EF-1α"
     promoter        2808..2855
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             2874..3245
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     terminator      3314..3561
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3636..4224)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

This page is informational only.