Basic Vector Information
- Vector Name:
- pIZ-OpIE-EGFP-MCS-V5-His
- Antibiotic Resistance:
- Zeocin
- Length:
- 3599 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Insect cells
- Selection Marker:
- Zeo
- Promoter:
- OpIE-2
- 5' Primer:
- EGFP-F3
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pIZ-OpIE-EGFP-MCS-V5-His vector Map
pIZ-OpIE-EGFP-MCS-V5-His vector Sequence
LOCUS . 3599 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 5..552
/label="OpIE-2 promoter"
/note="strong constitutive baculovirus promoter for insect
cell expression"
CDS 579..1295
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
CDS 1437..1454
/label="6xHis"
/note="6xHis affinity tag"
polyA_signal 1472..1601
/label="OpIE-2 poly(A) signal"
/note="baculovirus polyadenylation signal"
rep_origin complement(1729..2317)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 2965..3012
/label="EM7 promoter"
/note="synthetic bacterial promoter"
CDS 3032..3403
/label="BleoR"
/note="antibiotic-binding protein"
polyA_signal 3470..3604
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
This page is informational only.