Basic Vector Information
- Vector Name:
- LeGO-iG2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7823 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Promoter:
- SFFV
- 5' Primer:
- SFFV-F1
- Growth Temperature:
- 37℃
LeGO-iG2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
LeGO-iG2 vector Sequence
LOCUS V017430 7823 bp DNA circular SYN 01-JAN-1980
DEFINITION Exported.
ACCESSION V017430
VERSION V017430
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 7823)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..7823
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 238..617
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 618..820
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 835..1015
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1684..1917
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2102..2146
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 2295..2336
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 2444..2561
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind 2620..2636
/label="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
promoter 2778..3184
/label="SFFV promoter"
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
misc_feature 3270..3856
/label="IRES2"
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 3857..4573
/label="EGFP"
/note="enhanced GFP"
protein_bind complement(4623..4656)
/label="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
misc_feature 4712..5300
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5303..5319)
/label="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(6071..6659)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6833..7690)
/label="AmpR"
/note="beta-lactamase"
promoter complement(7691..7795)
/label="AmpR promoter"
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