Basic Vector Information
- Vector Name:
- pMJ329: pCMV-MmeFz2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5150 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CMV
- 5' Primer:
- CMV-F
- Growth Temperature:
- 37℃
pMJ329: pCMV-MmeFz2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMJ329: pCMV-MmeFz2 vector Sequence
LOCUS 62056_16965 5150 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5150) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..5150 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" polyA_signal 2161..2216 /label=beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(2577..2593) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(2601..2617) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2625..2655) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(2670..2691) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2749..2945 /label=SV40 promoter /note="SV40 early promoter" polyA_signal 2951..3085 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(3324..3912) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4086..4943) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4944..5048) /label=AmpR promoter enhancer 5079..5150 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer"
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