Basic Vector Information
- Vector Name:
- GST-BHMT-I/G
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7832 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CMV
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
GST-BHMT-I/G vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
GST-BHMT-I/G vector Sequence
LOCUS 62056_1076 7832 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7832)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..7832
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal complement(293..427)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(490..1206)
/label=EGFP
/note="enhanced GFP"
misc_feature complement(1209..1770)
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS complement(1811..2998)
/gene="BHMT"
/label=BHMT
/note="Betaine--homocysteine S-methyltransferase 1 from
Homo sapiens. Accession#: Q93088"
CDS complement(2993..3646)
/label=GST
/note="glutathione S-transferase from Schistosoma
japonicum"
promoter complement(3726..3744)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
promoter complement(3975..4178)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer complement(4179..4558)
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind complement(4587..4603)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4611..4627)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4635..4665)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4680..4701)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4989..5577)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5751..6608)
/label=AmpR
/note="beta-lactamase"
promoter complement(6609..6713)
/label=AmpR promoter
rep_origin complement(6995..7450)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 7663..7679
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(join(7699..7832,1..224))
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
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