CaV2.1 (pcDNA6) vector (V017341)

MSDS

Basic Vector Information

Vector Name:: CaV2.1 (pcDNA6)

Antibiotic Resistance:: Ampicillin

Length:: 12495 bp

Type:: Protein expression

Replication origin:: ori

Host:: Mammalian cells

Selection Marker:: Blast

Promoter:: SV40

5' Primer:: CMV-F

Growth Temperature:: 37℃

CaV2.1 (pcDNA6) vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

CaV2.1 (pcDNA6) vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_486       12495 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12495)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             755..7273
                     /gene="Cacna1a"
                     /label=Cacna1a
                     /note="Voltage-dependent P/Q-type calcium channel subunit 
                     alpha-1A from Rattus norvegicus. Accession#: P54282"
     CDS             8126..8167
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             8177..8194
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    8223..8447
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8493..8921
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8935..9264
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        9312..9359
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             9378..9773
                     /label=BSD
                     /note="blasticidin S deaminase"
     promoter        complement(10152..10182)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10197..10218)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10506..11094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11268..12125)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(12126..12230)
                     /label=AmpR promoter

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