Basic Vector Information
- Vector Name:
- Flag Nprl2 pRK5
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5861 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- SP6
- 5' Primer:
- CMV-F
- Growth Temperature:
- 37℃
Flag Nprl2 pRK5 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Flag Nprl2 pRK5 vector Sequence
LOCUS 62056_801 5861 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5861)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..5861
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 815..833
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
CDS 924..947
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
CDS 972..2111
/gene="NPRL2"
/label=NPRL2
/note="GATOR1 complex protein NPRL2 from Homo sapiens.
Accession#: Q8WTW4"
polyA_signal 2161..2295
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 2364..2721
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2741..2757)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 2970..3425
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3707..3811
/label=AmpR promoter
CDS 3812..4669
/label=AmpR
/note="beta-lactamase"
rep_origin 4843..5431
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 5719..5740
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5755..5785
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5793..5809
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5817..5833
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
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