Basic Vector Information
- Vector Name:
- pN565
- Antibiotic Resistance:
- Streptomycin
- Length:
- 9856 bp
- Type:
- Protein expression
- Replication origin:
- pSa ori
- Host:
- E. coli
- Copy Number:
- Low
- Promoter:
- T7
- Growth Temperature:
- 37℃
pN565 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pN565 vector Sequence
LOCUS 62056_17710 9856 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9856)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..9856
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 127..2772
/note="T7 RNA polymerase from Escherichia phage T7.
Accession#: P00573"
misc_feature 2776..2796
/label=BioBrick suffix
/note="universal suffix for all parts"
terminator 2832..2863
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
CDS 3725..4693
/label=RepA
/note="replication protein of plasmid pSa"
rep_origin 4733..5168
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
CDS 6843..7631
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
terminator 7792..7851
/label=his operon terminator
/note="This putative transcriptin terminator from the E.
coli his operon has a 2-bp deletion introduced during
synthesis. Its efficiency has not been determined."
terminator complement(7911..7940)
/label=T3Te terminator
/note="phage T3 early transcription terminator"
CDS complement(8046..9125)
/label=lacI
/note="lac repressor"
promoter complement(9126..9203)
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
promoter 9722..9750
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind complement(9756..9775)
/label=lac operator (symmetric)
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG). The
symmetric lac operator was optimized for tight binding of
lac repressor."
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