Basic Vector Information
- Vector Name:
- pLV-mTurquoise2-Caspase-1-mNeonGreen
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9378 bp
- Type:
- Gene expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Promoter:
- RSV
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pLV-mTurquoise2-Caspase-1-mNeonGreen vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV-mTurquoise2-Caspase-1-mNeonGreen vector Sequence
LOCUS V017242 9378 bp DNA circular SYN 01-JAN-1980 DEFINITION Exported. ACCESSION V017242 VERSION V017242 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 9378) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..9378 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 228..353 /label="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" misc_feature 846..1079 /label="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1264..1308 /label="gp41 peptide" /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 1457..1498 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" /label="Protein Tat" misc_feature 1575..1691 /label="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1" enhancer 1714..2017 /label="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 2018..2221 /label="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" CDS 2298..3014 /label="mTurquoise2" /note="enhanced monomeric variant of CFP (Goedhart et al., 2012)" CDS 3036..4247 /gene="CASP1" /label="Caspase-1" /note="Caspase-1 from Homo sapiens. Accession#: P29466" CDS 4278..4985 /label="mNeonGreen" /note="bright monomeric yellow-green fluorescent protein derived from LanYFP (Shaner et al., 2013)" misc_feature 4995..5583 /label="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 5670..5903 /label="3' LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 5975..6109 /label="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin 6136..6271 /label="SV40 ori" /note="SV40 origin of replication" promoter complement(6292..6310) /label="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6320..6336) /label="M13 fwd" /note="common sequencing primer, one of multiple similar variants" rep_origin 6478..6933 /label="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 6959..7063 /label="AmpR promoter" CDS 7064..7921 /label="AmpR" /note="beta-lactamase" rep_origin 8095..8683 /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 8971..8992 /label="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." promoter 9007..9037 /label="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 9045..9061 /label="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9069..9085 /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" promoter 9106..9124 /label="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" promoter 9152..9378 /label="RSV promoter" /note="Rous sarcoma virus enhancer/promoter"
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