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ELONGIN BC (XX01TCEB1A-c001) vector (V017233)

Basic Vector Information

Vector Name:
ELONGIN BC (XX01TCEB1A-c001)
Antibiotic Resistance:
Chloramphenicol
Length:
4587 bp
Type:
Gene expression
Replication origin:
p15A ori
Host:
E. coli
Copy Number:
Low
Promoter:
T7
Growth Strain(s):
DH5a
Growth Temperature:
37℃

ELONGIN BC (XX01TCEB1A-c001) vector Vector Map

ELONGIN BC (XX01TCEB1A-c001)4587 bp600120018002400300036004200lac operatorRBSELOBT7 promoterS-TagT7 terminatorCmRcat promoterp15A oriCAP binding sitelacIlacI promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

ELONGIN BC (XX01TCEB1A-c001) vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_686        4587 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             71..424
                     /gene="ELOB"
                     /label=ELOB
                     /note="Elongin-B from Homo sapiens. Accession#: Q15370"
     promoter        502..520
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             943..987
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     terminator      1039..1086
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1310..1966)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(1967..2069)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(2595..3140)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     protein_bind    complement(3331..3352)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3368..4447)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(4448..4525)
                     /label=lacI promoter

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