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BCR/ABL P190 transgenic construct vector (V017227)

Basic Vector Information

Vector Name:
BCR/ABL P190 transgenic construct
Antibiotic Resistance:
Ampicillin
Length:
13290 bp
Type:
Gene expression
Replication origin:
ori
Host:
Mammalian cells
Promoter:
T3
Growth Strain(s):
DH5a
Growth Temperature:
37℃

BCR/ABL P190 transgenic construct vector Vector Map

BCR/ABL P190 transgenic construct13290 bp600120018002400300036004200480054006000660072007800840090009600102001080011400120001260013200AmpRAmpR promoterf1 oriT7 promoterMCST3 promoterlac promoterCAP binding siteori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

BCR/ABL P190 transgenic construct vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_426       13290 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13290)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(174..1031)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1032..1136)
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1782..1800
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(1809..1916)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(12261..12279)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(12348..12378)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12393..12414)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(12702..13290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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