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pNO183 vector (V017192)

Basic Vector Information

Vector Name:
pNO183
Antibiotic Resistance:
Chloramphenicol
Length:
5684 bp
Type:
Protein expression
Replication origin:
ori
Host:
E. coli
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pNO183 vector Vector Map

pNO1835684 bp60012001800240030003600420048005400lambda t0 terminatorCmRRpBphP1rrnB T1 terminatorT7Te terminatorori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNO183 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_18180        5684 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(181..275)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(299..955)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2669..3610
                     /codon_start=1
                     /label=RpBphP1
                     /note="RpBphP1 is a bacterial phytochrome photoreceptor
                     derived from Rhodopseudomonas palustris and used as a 
                     template in the engineering of the miRFP family of 
                     near-infrared fluorescent proteins."
                     /translation="MAGHASGSPAFGTADLSNCEREEIHLAGSIQPHGALLVVSEPDHR
                     IIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEY
                     DGLMHRPPEGGLIIELERAGPPIDLSGTLAPALERIRTAGSLRALCDDTALLFQQCTGY
                     DRVMVYRFDEQGHGEVFSERHVPGLESYFGNRYPSSDIPQMARRLYERQRVRVLVDVSY
                     QPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQYLKNMGVRATLVVSLVVGGKLWGLVA
                     CHHYLPRFIHFELRAICELLAEAIATRITALES"
     terminator      4873..4944
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4960..4987
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(join(5189..5684,1..93))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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