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pMV306hsp+LuxG13 vector (V017191)

Basic Vector Information

Vector Name:
pMV306hsp+LuxG13
Antibiotic Resistance:
Kanamycin
Length:
10541 bp
Type:
Protein expression
Replication origin:
ori
Host:
E. coli
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pMV306hsp+LuxG13 vector Vector Map

pMV306hsp+LuxG1310541 bp5001000150020002500300035004000450050005500600065007000750080008500900095001000010500oriLuxALuxBLuxCLuxDLuxErrnB T1 terminatorIntegraseKanR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMV306hsp+LuxG13 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V017191                10541 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017191
VERSION     V017191
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10541)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      109..697
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             1367..2446
                     /label="LuxA"
                     /note="LuxA luciferase subunit"
     CDS             2464..3444
                     /label="LuxB"
                     /note="LuxB luciferase subunit"
     CDS             3934..5373
                     /label="LuxC"
                     /note="LuxC fatty acid reductase"
     CDS             5389..6309
                     /label="LuxD"
                     /note="LuxD acyltransferase"
     CDS             6337..7446
                     /label="LuxE"
                     /note="LuxE"
     terminator      7510..7556
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             8089..9201
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     CDS             9504..10316
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"

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