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GFP-MAP1B vector (V017190)

Basic Vector Information

Vector Name:
GFP-MAP1B
Antibiotic Resistance:
Kanamycin
Length:
12115 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Selection Marker:
Neo/G418
Promoter:
CMV
Growth Strain(s):
DH5a
Growth Temperature:
37℃

GFP-MAP1B vector Vector Map

GFP-MAP1B12115 bp60012001800240030003600420048005400600066007200780084009000960010200108001140012000CMV enhancerCMV promoterEGFPMap1bSV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRHSV TK poly(A) signalori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

GFP-MAP1B vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_1056       12115 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12115)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             620..1336
                     /label=EGFP
                     /note="enhanced GFP"
     CDS             1382..8773
                     /gene="Map1b"
                     /label=Map1b
                     /note="Microtubule-associated protein 1B from Mus musculus.
                     Accession#: P14873"
     polyA_signal    8910..9031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(9038..9493)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        9520..9624
                     /label=AmpR promoter
     promoter        9626..9983
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             10018..10809
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    11044..11091
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11420..12008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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