Basic Vector Information
- Vector Name:
- MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 6699 bp
- Type:
- Protein expression
- Replication origin:
- CloDF13 ori
- Host:
- E. coli
- Promoter:
- araBAD
- Growth Strain(s):
- Stable
- Growth Temperature:
- 37℃
MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG vector Sequence
LOCUS 62056_1665 6699 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6699) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..6699 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(39..777) /direction=LEFT /label=CloDF13 ori /note="Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins." terminator complement(870..913) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(930..1805) /label=araC /note="L-arabinose regulatory protein" promoter 1832..2116 /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" CDS 2172..2897 /gene="dnaQ" /label=dnaQ /note="DNA polymerase III subunit epsilon from Escherichia coli (strain K12). Accession#: P03007" CDS 2935..3765 /gene="dam" /label=dam /note="DNA adenine methylase from Escherichia coli (strain K12). Accession#: P0AEE8" CDS 3803..4342 /gene="seqA" /label=seqA /note="Negative modulator of initiation of replication from Escherichia coli (strain K12). Accession#: P0AFY8" CDS 4380..4904 /gene="mprA" /label=mprA /note="Transcriptional repressor MprA from Escherichia coli (strain K12). Accession#: P0ACR9" CDS 4942..5190 /label=UGI /note="uracil-DNA glycosylase inhibitor from a Bacillus subtilis bacteriophage (Mol et al., 1995)" CDS 5229..5849 /label=PmCDA1 /note="cytidine deaminase from the sea lamprey Petromyzon marinus (Rogozin et al., 2007)" terminator complement(5879..5910) /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" CDS complement(5929..6585) /label=CmR /note="chloramphenicol acetyltransferase"
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