Basic Vector Information
- Vector Name:
- pLenti-DsRed_IRES_SNCA:EGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10354 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pLenti-DsRed_IRES_SNCA:EGFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLenti-DsRed_IRES_SNCA:EGFP vector Sequence
LOCUS 62056_13830 10354 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10354)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..10354
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 376..633
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 680..805
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1302..1535
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1720..1764
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1913..1954
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
misc_feature 2057..2174
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 2226..2529
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2530..2733
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2843..3517
/label=DsRed-Express
/note="rapidly maturing tetrameric variant of DsRed
fluorescent protein (Bevis and Glick, 2002)"
misc_feature 3546..4130
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 4571..5287
/label=EGFP
/note="enhanced GFP"
promoter complement(5311..5329)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 5339..5814
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 5835..6626
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
misc_feature 6743..7331
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 7406..7639
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter 7844..7948
/label=AmpR promoter
CDS 7949..8806
/label=AmpR
/note="beta-lactamase"
rep_origin 8980..9568
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 9979..10000
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
LTR 10000..10354
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
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