Basic Vector Information
- Vector Name:
- CMV-SEAP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6034 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CMV
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
CMV-SEAP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
CMV-SEAP vector Sequence
LOCUS 62056_571 6034 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6034)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6034
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(35..1552)
/codon_start=1
/label=SEAP
/note="secreted alkaline phosphatase from human placenta"
/translation="MLGPCMLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAA
KKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKT
YNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGK
SVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVIL
GGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLD
PSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHG
HHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGL
APGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGED
VAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
promoter complement(1717..1920)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer complement(1921..2300)
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter complement(2534..2552)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2559..2575)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 2717..3169
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3195..3299
/label=AmpR promoter
CDS 3300..4157
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4331..4919
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 5207..5228
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5243..5273
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5281..5297
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5305..5321
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 5342..5360
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
polyA_signal complement(5406..5540)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
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