CRISPR-SP-Cas9 reporter vector (V017149)

Basic Vector Information

Vector Name:
CRISPR-SP-Cas9 reporter
Antibiotic Resistance:
8960 bp
Gene knockout
Replication origin:
Mammalian cells, Lentivirus
Selection Marker:
Growth Strain(s):
Growth Temperature:

CRISPR-SP-Cas9 reporter vector Vector Map

CRISPR-SP-Cas9 reporter8960 bp400800120016002000240028003200360040004400480052005600600064006800720076008000840088003' LTRHIV-1 PsiRREgp41 peptideProtein TatcPPT/CTSEF-1-alpha promoterHAdTomatoIRES2PuroRWPRE5' LTR (truncated)AmpR promoterAmpRoriCAP binding sitelac promoterlac operatorM13 rev

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

CRISPR-SP-Cas9 reporter vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V017149                 8960 bp    DNA     circular SYN 01-JAN-1980
VERSION     V017149
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8960)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2058..2175
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2223..3401
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3421..3447
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             3503..4201
                     /note="dimeric variant of DsRed fluorescent protein (Shaner
                     et al., 2004)"
     misc_feature    4207..4785
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4789..5385
                     /note="puromycin N-acetyltransferase"
     misc_feature    5402..5990
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6138..6318
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        6451..6555
                     /label="AmpR promoter"
     CDS             6556..7413
     rep_origin      7587..8175
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
     protein_bind    8586..8607
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8622..8652
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8660..8676
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8684..8700
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar

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