Basic Vector Information
- Vector Name:
- Cas12f-GE ver4.1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6802 bp
- Type:
- Gene knockout
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CBh
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
Cas12f-GE ver4.1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Cas12f-GE ver4.1 vector Sequence
LOCUS 62056_476 6802 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6802) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..6802 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 6..246 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" enhancer 545..830 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer" promoter 832..1106 /label=chicken beta-actin promoter CDS 1358..1378 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" CDS 1403..2989 /gene="cas12f" /label=cas12f /note="CRISPR-associated endodeoxyribonuclease Cas12f1 from Uncultured archaeon. Accession#: A0A482D308" CDS 3107..3820 /label=EGFP /note="enhanced GFP" polyA_signal 3854..4061 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 4070..4210 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" rep_origin 4285..4740 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 5022..5126 /label=AmpR promoter CDS 5127..5984 /label=AmpR /note="beta-lactamase" rep_origin 6158..6746 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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