Cas12f-GE ver4.0 vector (V017095)

MSDS

Basic Vector Information

Vector Name:: Cas12f-GE ver4.0

Antibiotic Resistance:: Ampicillin

Length:: 6832 bp

Type:: Gene knockout

Replication origin:: ori

Host:: Mammalian cells

Promoter:: CBh

Growth Strain(s):: DH5a

Growth Temperature:: 37℃

Cas12f-GE ver4.0 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Cas12f-GE ver4.0 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_471        6832 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..246
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     enhancer        572..857
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer;
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        859..1136
                     /label=chicken beta-actin promoter
     CDS             1388..1408
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             1433..3019
                     /gene="cas12f"
                     /label=cas12f
                     /note="CRISPR-associated endodeoxyribonuclease Cas12f1 from
                     Uncultured archaeon. Accession#: A0A482D308"
     CDS             3137..3850
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    3884..4091
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4100..4240
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4315..4770
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5052..5156
                     /label=AmpR promoter
     CDS             5157..6014
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6188..6776
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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