Basic Vector Information
- Vector Name:
- pLXSN16E6E7
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6716 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Retrovirus
- Selection Marker:
- Neo/G418
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pLXSN16E6E7 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLXSN16E6E7 vector Sequence
LOCUS 62056_16375 6716 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6716) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..6716 /mol_type="other DNA" /organism="synthetic DNA construct" LTR 176..763 /label=5' LTR /note="long terminal repeat from Moloney murine sarcoma virus" misc_feature 826..1025 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1226..1642 /codon_start=1 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA" CDS 1689..2162 /codon_start=1 /label=HPV16E6 /note="Human papillomavirus type 16 transforming protein E6 gene" /translation="MHQKRTAMFQDPQERPRKLPQLCTELQTTIHDIILECVYCKQQLL RREVYDFAFRDLCIVYRDGNPYAVCDKCLKFYSKISEYRHYCYSLYGTTLEQQYNKPLC DLLIRCINCQKPLCPEEKQRHLDKKQRFHNIRGRWTGRCMSCCRSSRTRRETQL" CDS 2168..2461 /codon_start=1 /label=HPV16E7 /note="Human papillomavirus type 16 transforming protein E7 gene" /translation="MHGDTPTLHEYMLDLQPETTDLYCYEQLNDSSEEEDEIDGPAGQA EPDRAHYNIVTFCCKCDSTLRLCVQSTHVDIRTLEDLLMGTLGIVCPICSQKP" promoter 2517..2846 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 2906..3697 /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAXXXLXXEFF" LTR 3762..4355 /label=LTR /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 4566..4706 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(4892..5480) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5654..6511) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(6512..6616) /label=AmpR promoter
This page is informational only.