pMS2M-PH-gagpol-D64V vector (V017074)

MSDS

Basic Vector Information

Vector Name:: pMS2M-PH-gagpol-D64V

Antibiotic Resistance:: Ampicillin

Length:: 10711 bp

Type:: Protein expression

Replication origin:: ori

Host:: Mammalian cells

Growth Strain(s):: Stbl3

Growth Temperature:: 37℃

pMS2M-PH-gagpol-D64V vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMS2M-PH-gagpol-D64V vector Sequence

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Download GeneBank File(.gb)

LOCUS       62056_17275       10711 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             866..1252
                     /label=MS2-N55K
                     /note="bacteriophage MS2 coat protein"
     misc_feature    1277..1342
                     /label=MCS
                     /note="multiple cloning site"
     CDS             1886..3421
                     /gene="gag"
                     /label=gag
                     /note="Gag polyprotein from Human immunodeficiency virus
                     type 1 group M subtype B (isolate BH10). Accession#: 
                     P03347"
     CDS             3265..6225
                     /label=HIV-1 pol
                     /note="pol protein from human immunodeficiency virus 1"
     promoter        complement(6265..6283)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    6309..6533
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6579..7006
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7020..7349
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7416..8207
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    8384..8517
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(8602..8632)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8647..8668)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8956..9544)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9718..10575)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10576..10680)
                     /label=AmpR promoter

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