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pN094 vector (V017025)

Basic Vector Information

Vector Name:
pN094
Antibiotic Resistance:
Ampicillin
Length:
6659 bp
Type:
Protein expression
Replication origin:
oriV
Host:
Plants
Promoter:
CaMV 35S
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pN094 vector Vector Map

pN0946659 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600LB T-DNA repeatoriVAmpR promoterAmpRoritrfARB T-DNA repeatOCS terminatornpgATMV OmegaCaMV 35S promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pN094 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_17695        6659 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(76..100)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      184..893
                     /label=oriV
                     /note="incP origin of replication"
     promoter        910..1014
                     /label=AmpR promoter
     CDS             1015..1872
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2046..2634
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             2970..4115
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     misc_feature    complement(4274..4298)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(4347..5054)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(5082..6113)
                     /gene="npgA"
                     /label=npgA
                     /note="4'-phosphopantetheinyl transferase NpgA from
                     Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 
                     112.46 / NRRL 194 / M139). Accession#: G5EB87"
     misc_feature    complement(6122..6175)
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(6204..6549)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"

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