Basic Vector Information
- Vector Name:
- MSP832
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10017 bp
- Type:
- Protein expression
- Replication origin:
- pSC101 ori
- Host:
- E. coli
- Promoter:
- T7
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 30℃
MSP832 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
MSP832 vector Sequence
LOCUS 62056_1690 10017 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10017) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..10017 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(4..951) /label=Rep101 /note="RepA protein needed for replication with the pSC101 origin" rep_origin complement(999..1221) /direction=LEFT /label=pSC101 ori /note="low-copy replication origin that requires the Rep101 protein" promoter 1785..1803 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1985..3256 /label=M13 gene III /note="pIII" CDS 3259..4338 /label=LuxA /note="LuxA luciferase subunit" CDS 4402..5379 /label=LuxB /note="LuxB luciferase subunit" CDS complement(5482..8127) /note="T7 RNA polymerase from Escherichia phage T7. Accession#: P00573" CDS complement(8212..8664) /label=T7 lysozyme /note="lysozyme from bacteriophage T7" terminator complement(8849..8895) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(8949..9806) /label=AmpR /note="beta-lactamase" promoter complement(9807..9911) /label=AmpR promoter
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