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MSP955 vector (V016994)

Basic Vector Information

Vector Name:
MSP955
Antibiotic Resistance:
Ampicillin
Length:
10017 bp
Type:
Protein expression
Replication origin:
pSC101 ori
Host:
E. coli
Promoter:
T7
Growth Strain(s):
DH5a
Growth Temperature:
30℃

MSP955 vector Vector Map

MSP95510017 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000Rep101pSC101 oriT7 promoterpIIILuxALuxBT7 RNA polymerase from Escherichia phage T7. Accession#: P00573TEV siteT7 lysozymerrnB T1 terminatorAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

MSP955 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_1695       10017 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10017)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..951)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(999..1221)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     promoter        1785..1803
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1985..3256
                     /label=M13 gene III
                     /note="pIII"
     CDS             3259..4338
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             4402..5379
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             complement(5482..8127)
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
     CDS             complement(8161..8181)
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             complement(8212..8664)
                     /label=T7 lysozyme
                     /note="lysozyme from bacteriophage T7"
     terminator      complement(8849..8895)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(8949..9806)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9807..9911)
                     /label=AmpR promoter

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