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MP-P8 vector (V016992)

Basic Vector Information

Vector Name:
MP-P8
Antibiotic Resistance:
Chloramphenicol
Length:
6651 bp
Type:
Protein expression
Replication origin:
CloDF13 ori
Host:
E. coli
Promoter:
araBAD
Growth Strain(s):
DH5a
Growth Temperature:
37℃

MP-P8 vector Vector Map

MP-P86651 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600CloDF13 orirrnB T1 terminatoraraCaraBAD promoterdnaQdamseqAmprAUGIAIDtonB terminatorCmR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

MP-P8 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_1655        6651 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(39..777)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     terminator      complement(870..913)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(930..1805)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        1832..2116
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             2166..2894
                     /gene="dnaQ"
                     /label=dnaQ
                     /note="DNA polymerase III subunit epsilon from Escherichia
                     coli (strain K12). Accession#: P03007"
     CDS             2926..3759
                     /gene="dam"
                     /label=dam
                     /note="DNA adenine methylase from Escherichia coli (strain
                     K12). Accession#: P0AEE8"
     CDS             3791..4333
                     /gene="seqA"
                     /label=seqA
                     /note="Negative modulator of initiation of replication from
                     Escherichia coli (strain K12). Accession#: P0AFY8"
     CDS             4365..4892
                     /gene="mprA"
                     /label=mprA
                     /note="Transcriptional repressor MprA from Escherichia coli
                     (strain K12). Accession#: P0ACR9"
     CDS             4924..5175
                     /label=UGI
                     /note="uracil-DNA glycosylase inhibitor from a Bacillus
                     subtilis bacteriophage (Mol et al., 1995)"
     CDS             5208..5801
                     /label=AID
                     /note="human activation-induced cytidine deaminase
                     (Dickerson et al., 2003)"
     terminator      5831..5862
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     CDS             complement(5881..6537)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"

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