Basic Vector Information
- Vector Name:
- pLVX-IRES-Gαi*-BERKY3
- Antibiotic Resistance:
- Ampicillin
- Length:
- 11726 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Selection Marker:
- Hyg
- Promoter:
- CMV
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pLVX-IRES-Gαi*-BERKY3 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLVX-IRES-Gαi*-BERKY3 vector Sequence
LOCUS 62056_16145 11726 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11726) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..11726 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" CDS 1233..1253 /label=TEV site /note="tobacco etch virus (TEV) protease recognition and cleavage site" CDS 1509..2225 /label=Citrine /note="enhanced variant of YFP (Heikal et al., 2001)" misc_feature 3850..4436 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 4462..5478 /label=HygR /note="aminoglycoside phosphotransferase from E. coli" misc_feature 5495..6083 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 6290..6923 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" promoter complement(7099..7129) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(7144..7165) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(7453..8041) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8215..9072) /label=AmpR /note="beta-lactamase" promoter complement(9073..9177) /label=AmpR promoter polyA_signal 9225..9359 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" misc_feature 10207..10332 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 10829..11062 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 11247..11291 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 11440..11481 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" misc_feature 11553..11670 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1"
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