Basic Vector Information
- Vector Name:
- MG15-Luc (mut p53 binding sites)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6013 bp
- Type:
- Signal transduction
- Replication origin:
- ori
- Host:
- Mammalian cells
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
MG15-Luc (mut p53 binding sites) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
MG15-Luc (mut p53 binding sites) vector Sequence
LOCUS 62056_1605 6013 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6013) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..6013 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 480..2129 /codon_start=1 /label=luciferase /note="firefly luciferase" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL" intron 2263..2328 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 2458..2478 /codon_start=1 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" /translation="PKKKRKV" polyA_signal 2903..3037 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter complement(3087..3105) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(3126..3142) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(3150..3166) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3174..3204) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(3219..3240) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(3580..4168) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4342..5199) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5200..5304) /label=AmpR promoter rep_origin complement(5330..5785) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 5927..5943 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 5950..5968 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 5994..6010 /label=KS primer /note="common sequencing primer, one of multiple similar variants"
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