Basic Vector Information
- Vector Name:
- GW1-PercevalHR
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7010 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CMV
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
GW1-PercevalHR vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
GW1-PercevalHR vector Sequence
LOCUS 62056_1096 7010 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7010) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..7010 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 381..632 /codon_start=1 /label=VC155 /note="C-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH YLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" polyA_signal 2050..2184 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2208..2224) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(2232..2248) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2256..2286) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(2301..2322) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(2610..3198) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3372..4229) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4883..4899 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 5448..5827 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 5828..6031 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" intron 6167..6993 /label=CMV intron A /note="human cytomegalovirus intron A (Chapman et al., 1991)"
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