Basic Vector Information
- Vector Name:
- Flag-SENP1m
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6605 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CMV
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
Flag-SENP1m vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Flag-SENP1m vector Sequence
LOCUS 62056_811 6605 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6605)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6605
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 500..521
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 536..566
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 574..590
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 598..614
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 628..646
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(685..1014)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
polyA_signal complement(1043..1665)
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
CDS complement(1691..3622)
/gene="SENP1"
/label=SENP1
/note="Sentrin-specific protease 1 from Homo sapiens.
Accession#: Q9P0U3"
CDS complement(3653..3676)
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
promoter complement(3710..3913)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer complement(3914..4293)
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind complement(4454..4470)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(4611..5066)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5093..5197
/label=AmpR promoter
CDS 5198..6055
/label=AmpR
/note="beta-lactamase"
rep_origin 6229..6605
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
This page is informational only.