pMIG-hNFATc2/C-AVITEV vector (V016686)

MSDS

Basic Vector Information

Vector Name:: pMIG-hNFATc2/C-AVITEV

Antibiotic Resistance:: Ampicillin

Length:: 9935 bp

Type:: Protein expression

Replication origin:: ori

Host:: Mammalian cells

Promoter:: MSCV

Growth Strain(s):: DH5a

Growth Temperature:: 37℃

pMIG-hNFATc2/C-AVITEV vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMIG-hNFATc2/C-AVITEV vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_16785        9935 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             43..560
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"
     misc_feature    624..965
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell
                     virus"
     CDS             1032..1448
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     CDS             1485..4259
                     /gene="NFATC2"
                     /label=NFATC2
                     /note="Nuclear factor of activated T-cells, cytoplasmic 2
                     from Homo sapiens. Accession#: Q13469"
     CDS             4266..4286
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             4299..4343
                     /label=AviTag(TM)
                     /note="peptide tag that allows for enzymatic biotinylation"
     misc_feature    4412..4991
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             4992..5708
                     /label=EGFP
                     /note="enhanced GFP"
     promoter        complement(6534..6564)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6579..6600)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6888..7476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7650..8507)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8508..8612)
                     /label=AmpR promoter
     promoter        8905..9234
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        9282..9329
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"

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