Basic Vector Information
- Vector Name:
- Flag-SENP1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6605 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CMV
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
Flag-SENP1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Flag-SENP1 vector Sequence
LOCUS 62056_806 6605 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6605) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..6605 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 107..128 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 235..253 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter complement(292..621) /label=SV40 promoter /note="SV40 enhancer and early promoter" polyA_signal complement(650..1272) /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" CDS complement(1298..3229) /gene="SENP1" /label=SENP1 /note="Sentrin-specific protease 1 from Homo sapiens. Accession#: Q9P0U3" CDS complement(3260..3283) /label=FLAG /note="FLAG(R) epitope tag, followed by an enterokinase cleavage site" promoter complement(3317..3520) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" enhancer complement(3521..3900) /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" primer_bind complement(4061..4077) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin complement(4218..4673) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 4700..4804 /label=AmpR promoter CDS 4805..5662 /label=AmpR /note="beta-lactamase" rep_origin 5836..6424 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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