Basic Vector Information
- Vector Name:
- GOMO-GFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 11695 bp
- Type:
- Signal transduction
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- hDHFR-yFCU
- Promoter:
- PbHSP70
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
GOMO-GFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
GOMO-GFP vector Sequence
LOCUS 62056_1066 11695 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11695)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..11695
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(990..1697)
/label=mCherry
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
CDS 2762..3322
/gene="DHFR"
/label=DHFR
/note="Dihydrofolate reductase from Homo sapiens.
Accession#: P00374"
CDS 3365..3835
/gene="FCY1"
/label=FCY1
/note="Cytosine deaminase from Saccharomyces cerevisiae
(strain ATCC 204508 / S288c). Accession#: Q12178"
CDS 3836..4480
/gene="FUR1"
/label=FUR1
/note="Uracil phosphoribosyltransferase from Saccharomyces
cerevisiae (strain ATCC 204508 / S288c). Accession#:
P18562"
CDS complement(6199..6909)
/label=yeGFP
/note="yeast-enhanced green fluorescent protein"
promoter complement(8855..8873)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter complement(8942..8972)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(8987..9008)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(9296..9884)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10058..10915)
/label=AmpR
/note="beta-lactamase"
promoter complement(10916..11020)
/label=AmpR promoter
rep_origin complement(11046..11501)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 11643..11659
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 11669..11687
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
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