Basic Vector Information
- Vector Name:
- CaV1.3e[8a1131bΔ3242a] mut
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10850 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Blast
- Promoter:
- EM7
- Growth Strain(s):
- Top10F'
- Growth Temperature:
- 37℃
CaV1.3e[8a1131bΔ3242a] mut vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
CaV1.3e[8a1131bΔ3242a] mut vector Sequence
LOCUS 62056_481 10850 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10850) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..10850 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 6715..6756 /codon_start=1 /label=V5 tag /note="epitope tag from simian virus 5" /translation="GKPIPNPLLGLDST" CDS 6766..6783 /codon_start=1 /label=6xHis /note="6xHis affinity tag" /translation="HHHHHH" polyA_signal 6812..7036 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7082..7510 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 7524..7853 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 7901..7948 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 7967..8362 /codon_start=1 /label=BSD /note="blasticidin S deaminase" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" polyA_signal 8523..8656 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter complement(8741..8771) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(8786..8807) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(9095..9683) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9857..10714) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(10715..10819) /label=AmpR promoter
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