Basic Vector Information
This vector seems to be unstable. The Sanger sequencing was correct, but the restriction digestion was not. There is no good signal from the third-generation sequencing.
- Vector Name:
- pGTMV
- Antibiotic Resistance:
- Kanamycin
- Length:
- 9669 bp
- Type:
- Protein expression
- Replication origin:
- pSa ori
- Host:
- Plants
- Copy Number:
- Low
- Promoter:
- 35s
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pGTMV vector Map
pGTMV vector Sequence
LOCUS Exported 9669 bp DNA circular SYN 05-SEP-2024
DEFINITION Exported.
ACCESSION V016602
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9669)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 9669)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9669
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 20..3508
/codon_start=1
/product="Replicase [Tobacco mosaic virus]"
/label=Replicase
/translation="MTHNPTILRKTLPLYKEVHFIWRGIFTTITNNNKQQTTLQLLFTI
TMAYTQTATTSALLDTVRGNNSLVNDLAKRRLYDTAVEEFNARDRRPKVNFSKVISEEQ
TLIATRAYPEFQITFYNTQNAVHSLAGGLRSLELEYLMMQIPYGSLTYDIGGNFASHLF
KGRAYVHCCMPNLDVRDIMRHEGQKDSIELYLSRLERGGKTVPNFQKEAFDRYAEIPED
AVCHNTFQTCEHQPMQQSGRVYAIALHSIYDIPADEFGAALLRKNVHTCYAAFHFSENL
LLEDSCVNLDEINACFSRDGDKLTFSFASESTLNYCHSYSNILKYVCKTYFPASNREVY
MKEFLVTRVNTWFCKFSRIDTFLLYKGVAHKSVDSEQFYTAMEDAWHYKKTLAMCNSER
ILLEDSSSVNYWFPKMRDMVIVPLFDISLETSKRTRKEVLVSKDFVFTVLNHIRTYQAK
ALTYANVLSFVESIRSRVIINGVTARSEWDVDKSLLQSLSMTFYLHTKLAVLKDDLLIS
KFSLGSKTVCQHVWDEISLAFGNAFPSVKERLLNRKLIRVAGDALEIRVPDLYVTFHDR
LVTEYKASVDMPALDIRKKMEETEVMYNALSELSVLRESDKFDVDVFSQMCQSLEVDPM
TAAKVIVAVMSNESGLTLTFERPTEANVALALQDQEKASEGALVVTSREVEEPSMKGSM
ARGELQLAGLAGDHPESSYSKNEEIESLEQFHMATADSLIRKQMSSIVYTGPIKVQQMK
NFIDSLVASLSAAVSNLVKILKDTAAIDLETRQKFGVLDVASRKWLIKPTAKSHAWGVV
ETHARKYHVALLEYDEQGVVTCDDWRRVAVSSESVVYSDMAKLRTLRRLLRNGEPHVSS
AKVVLVDGVPGCGKTKEILSRVNFDEDLILVPGKQAAEMIRRRANSSGIIVATKDNVKT
VDSFMMNFGKSTRCQFKRLFIDEGLMLHTGCVNFLVAMSLCEIAYVYGDTQQIPYINRV
SGFPYPAHFAKLEVDEVETRRTTLRCPADVTHYLNRRYEGFVMSTSSVKKSVSQEMVGG
AAVINPISKPLHGKILTFTQSDKEALLSRGYSDVHTVHEVQGETYSDVSLVRLTPTPVS
IIAGDSPHVLVALSRHTCSLKYYTVVMDPLVSIIRDLEKLSSYLLDMYKVDAGTQ"
misc_feature 101..156
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 3584..5008
/codon_start=1
/product="Replicase [Tobacco mosaic virus]"
/label=Replicase
/translation="MQFYYDKCLPGNSTMMNNFDAVTMRLTDISLNVKDCILDMSKSVA
APKDQIKPLIPMVRTAAEMPRQTGLLENLVAMIKRNFNAPELSGIIDIENTASLVVDKF
FDSYLLKEKRKPNKNVSLFSRESLNRWLEKQEQVTIGQLADFDFVDLPAVDQYRHMIKA
QPKQKLDTSIQTEYPALQTIVYHSKKINAIFGPLFSELTRQLLDSVDSSRFLFFTRKTP
AQIEDFFGDLDSHVPMDVLELDISKYDKSQNEFHCAVEYEIWRRLGFEDFLGEVWKQGH
RKTTLKDYTAGIKTCIWYQRKSGDVTTFIGNTVIIAACLASMLPMEKIIKGAFCGDDSL
LYFPKGCEFPDVQHSANLMWNFEAKLFKKQYGYFCGRYVIHHDRGCIVYYDPLKLISKL
GAKHIKDWEHLEEFRRSLCDVAVSLNNCAYYTQLDDAVWEVHKTAPPGSFVYKSLVKYL
SDKVLFRSLFIDGSSC"
CDS 4992..5795
/gene="MP"
/label=Movement protein
/note="Movement protein from Tobacco mosaic virus (strain
vulgare). Accession#: P03583"
CDS 5801..6277
/gene="CP"
/label=Capsid protein
/note="Capsid protein from Tobacco mosaic virus (strain
vulgare). Accession#: P69687"
misc_feature 6774..6798
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(6917..7505)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7679..8491)
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 8782..9217
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
misc_feature 9368..9392
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
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