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pPH37 vector (V016592)

Basic Vector Information

Vector Name:
pPH37
Antibiotic Resistance:
Ampicillin
Length:
3612 bp
Type:
Gene knockout
Replication origin:
ori
Host:
Yeast
Selection Marker:
cytosine
Promoter:
TEF
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pPH37 vector Vector Map

pPH373612 bp60012001800240030003600TEF promoterCytosine deaminaseTEF terminatorT7 promoteroriAmpRAmpR promoterSP6 promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pPH37 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V016592                 3612 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V016592
VERSION     V016592
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        115..458
                     /label="TEF promoter"
                     /note="Ashbya gossypii TEF promoter"
     CDS             459..932
                     /gene="FCY1"
                     /label="Cytosine deaminase"
                     /note="Cytosine deaminase from Saccharomyces cerevisiae
                     (strain ATCC 204508 / S288c). Accession#: Q12178"
     terminator      941..1138
                     /label="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(1250..1268)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1526..2114)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2288..3145)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3146..3250)
                     /label="AmpR promoter"
     promoter        3596..3612
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"

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