RS213_ErbB-RASER1N-OFP vector (V016574)

LOCUS       V016574                 8785 bp    DNA     circular SYN 18-MAY-2019
DEFINITION  Exported.
ACCESSION   V016574
VERSION     V016574
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Chung HK, Zou X, Bajar BT, Brand VR, Huo Y, Alcudia JF, Ferrell JE
            Jr., Lin MZ.
  TITLE     A compact synthetic pathway rewires cancer signaling to therapeutic
            effector release
  JOURNAL   Science 364 (6439) (2019)
   PUBMED   31048459
REFERENCE   2  (bases 1 to 8785)
  AUTHORS   Chung HK, Lin MZ.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2019) Bioengineering, Stanford University, 269
            Campus Drive, CCRS2100, Stanford, CA 94305, United States
REFERENCE   3  (bases 1 to 8785)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Science 364
            (6439) (2019)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-APR-2019) Bioengineering, Stanford University, 269 Campus Drive,
            CCRS2100, Stanford, CA 94305, United States"
FEATURES             Location/Qualifiers
     source          1..8785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..564
                     /label="nonstandard type: promoter; CMV_imm early promoter
                     2"
     misc_feature    67..354
                     /label="CAG enhancer"
                     /note="CAG enhancer"
     misc_feature    521..541
                     /label="nonstandard type: primer_bind; CMV fwd primer"
     LTR             584..764
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    811..936
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1435..1668
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1853..1897
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2046..2087
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2195..2312
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2371..2684
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das
                     et al., 1988)"
     primer_bind     2701..2717
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2759..2792)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        2908..3211
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3212..3415
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     3414..3465
                     /label="p1317"
                     /note="p1317"
     misc_feature    3447..4004
                     /label="Shc-PTB"
                     /note="Shc-PTB"
     misc_feature    3810..3830
                     /label="HIF1a"
                     /note="HIF1a"
     CDS             4008..4031
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    4068..4646
                     /label="nonstandard type: Protease; NS3Tprotease"
     misc_feature    4227..4229
                     /label="nonstandard type: misc; T54"
     primer_bind     4394..4426
                     /label="p1278"
                     /note="p1278"
     misc_feature    complement(4412..4442)
                     /direction=LEFT
                     /label="nonstandard type: primer_bind_reverse; p1502 MreI
                     rev"
     misc_feature    4647..4712
                     /label="P2A"
                     /note="P2A"
     misc_feature    complement(4653..4712)
                     /direction=LEFT
                     /label="nonstandard type: primer_bind_reverse; p1401 P2A
                     reverse"
     CDS             4713..5363
                     /label="mKO2"
                     /note="monomeric Kusabira-Orange 2 fluorescent protein"
     misc_feature    5376..5417
                     /label="nonstandard type: epitope; V5"
     misc_feature    5418..5450
                     /label="nonstandard type: Substrate; substrate 4"
     misc_feature    complement(5451..5489)
                     /direction=LEFT
                     /label="nonstandard type: primer_bind_reverse; p1514 GGS
                     linker"
     misc_feature    5469..5852
                     /label="nonstandard type: gBLOCK; HKC gBLOCK-5 VAV1"
     misc_feature    5490..5822
                     /label="nonstandard type: SH2; VAV1"
     primer_bind     5823..5858
                     /label="p1511 GGs linker before CAAX"
                     /note="p1511 GGs linker before CAAX"
     misc_feature    5880..5939
                     /label="CAAX"
                     /note="CAAX"
     misc_feature    5943..5948
                     /label="EcoRV"
                     /note="EcoRV"
     misc_feature    5961..6549
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(6552..6568)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    6734..6755
                     /label="U3PPT"
                     /note="U3PPT"
     misc_feature    6734..6749
                     /label="cPPT(1)"
                     /note="cPPT(1)"
     misc_feature    6791..6971
                     /label="truncHIV-1 3 LTR(1)"
                     /note="truncHIV-1 3 LTR(1)"
     rep_origin      complement(7033..7621)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7795..8652)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8653..8757)
                     /label="AmpR promoter"