Basic Vector Information
- Vector Name:
- HITI-Lectin
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3732 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Gunadi A, Orchard N, Qu F, Finer J.
HITI-Lectin vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
HITI-Lectin vector Sequence
LOCUS 62056_1196 3732 bp DNA circular SYN 01-FEB-2020 DEFINITION Cloning vector HITI-Lectin, complete sequence. ACCESSION MN043932 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3732) AUTHORS Gunadi A, Orchard N, Qu F, Finer J. TITLE Enhanced CRISPR/Cas9 mediated homology-independent targeted integration in plant cotyledonary tissues JOURNAL Unpublished REFERENCE 2 (bases 1 to 3732) AUTHORS Gunadi A, Orchard N, Qu F, Finer J. TITLE Direct Submission JOURNAL Submitted (06-JUN-2019) Horticulture and Crop Science, The Ohio State University, 216 Williams Hall, 1680 Madison Ave., Wooster, OH 44691, USA REFERENCE 3 (bases 1 to 3732) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (06-JUN-2019) Horticulture and Crop Science, The Ohio State University, 216 Williams Hall, 1680 Madison Ave., Wooster, OH 44691, USA" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3732 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS 429..1145 /codon_start=1 /label=EGFP /note="enhanced GFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITHGMDELYK" terminator 1181..1433 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(1511..1527) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1535..1551) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1559..1589) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(1604..1625) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1913..2501) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2675..3532) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3533..3637) /label=AmpR promoter
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