pBAC_attBs_fle-3miR vector (V016529)

LOCUS       62056_3460        9937 bp DNA     circular SYN 27-JAN-2021
DEFINITION  Cloning vector pBAC_attBs_fle-3miR, complete sequence.
ACCESSION   MW147152
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Krzywinska E, Ferretti L, Li J, Li JC, Chen CH, Krzywinski J.
  TITLE     Femaleless Controls Sex Determination and Dosage Compensation 
            Pathways in Females of Anopheles Mosquitoes
  JOURNAL   Curr Biol (2021) In press
  PUBMED    33417880
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   Krzywinski J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2020) Vector Molecular Biology Group, The 
            Pirbright Institute, Ash Road, Pirbright GU24 0NF, United Kingdom
REFERENCE   3  (bases 1 to 9937)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol 
            (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-OCT-2020) Vector Molecular Biology Group, The Pirbright 
            Institute, Ash Road, Pirbright GU24 0NF, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..9937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    122..242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(274..307)
                     /label=attB
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et
                     al., 2000)"
     misc_feature    313..1139
                     /label=piggyBac right arm
                     /note="piggyBac right arm"
     protein_bind    complement(1148..1181)
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     CDS             1531..2187
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     rep_origin      2314..2902
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3658..3691
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     misc_feature    3702..4402
                     /label=piggyBac left arm
                     /note="piggyBac left arm"
     protein_bind    4408..4441
                     /label=attB
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et
                     al., 2000)"
     gene            4472..6434
                     /gene="AGAP001971"
                     /label=AGAP001971
     regulatory      4472..6434
                     /gene="AGAP001971"
                     /label=Anopheles gambiae polyubiquitin
                     /note="Anopheles gambiae polyubiquitin"
                     /regulatory_class="promoter"
     misc_RNA        6445..7092
                     /label=cluster of 3 microRNAs targeting the fle gene
                     /note="cluster of 3 microRNAs targeting the fle gene"
     regulatory      7106..7335
                     /label=SV40
                     /note="SV40"
                     /regulatory_class="terminator"
     gene            7349..7946
                     /gene="AGAP004395"
                     /label=AGAP004395
     regulatory      7349..7946
                     /gene="AGAP004395"
                     /regulatory_class="promoter"
     CDS             7953..8549
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     regulatory      8559..8788
                     /label=SV40
                     /note="SV40"
                     /regulatory_class="terminator"
     gene            8789..9217
                     /gene="AGAP003430"
                     /label=AGAP003430
     regulatory      8789..9217
                     /gene="AGAP003430"
                     /regulatory_class="promoter"
     CDS             9218..9934
                     /label=EGFP
                     /note="enhanced GFP"