pGBT-RP8.2 vector (V016511)

LOCUS       62056_11330        5985 bp DNA     circular SYN 25-AUG-2020
DEFINITION  Cloning vector pGBT-RP8.2, complete sequence.
ACCESSION   MT815591
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ichino N, Serres MR, Urban RM, Urban MD, Treichel AJ, Schaefbauer 
            KJ, Greif LE, Varshney GK, Skuster KJ, McNulty MS, Daby CL, Wang Y, 
            Liao HK, El-Rass S, Ding Y, Liu W, Anderson JL, Wishman MD, 
            Sabharwal A, Schimmenti LA, Sivasubbu S, Balciunas D, Hammerschmidt 
            M, Farber SA, Wen XY, Xu X, McGrail M, Essner JJ, Burgess SM, Clark 
            KJ, Ekker SC.
  TITLE     Building the vertebrate codex using the gene breaking protein trap 
            library
  JOURNAL   Elife 9 (2020) In press
  PUBMED    32779569
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Ichino N, Serres MR, Urban RM, Urban MD, Treichel AJ, Schaefbauer 
            KJ, Greif LE, Varshney GK, Skuster KJ, McNulty MS, Daby CL, Wang Y, 
            Liao HK, El-Rass S, Ding Y, Liu W, Anderson JL, Wishman MD, 
            Sabharwal A, Schimmenti LA, Sivasubbu S, Balciunas D, Hammerschmidt 
            M, Farber SA, Wen XY, Xu X, McGrail M, Essner JJ, Burgess SM, Clark 
            KJ, Ekker SC.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2020) Biochemistry and Molecular Biology, Mayo 
            Clinic, 200 First Street SW, Rochester, Olmsted, MN 55905, USA
REFERENCE   3  (bases 1 to 5985)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife 9 
            (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JUL-2020) Biochemistry and Molecular Biology, Mayo Clinic, 200 
            First Street SW, Rochester, Olmsted, MN 55905, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   6..196
                     /label=Tol2
                     /note="Tol2"
     promoter        complement(204..222)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_recomb     248..281
                     /label=loxP
                     /note="loxP"
     misc_feature    288..397
                     /note="derived from carp; contains beta actin splice
                     acceptor from intron 1/exon2 boundary"
     CDS             452..1123
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     misc_feature    1135..2310
                     /note="contains 3'UTR, poly(A) signal, and putative
                     boundary element from ocean pout (FRM- Pat Gibbs)"
     regulatory      2354..2714
                     /label=Xenopus gamma-crystallin
                     /note="Xenopus gamma-crystallin"
                     /regulatory_class="promoter"
     misc_feature    2743..2766
                     /note="derived from carp; contains beta actin non-coding
                     exon 1"
     misc_feature    2767..2964
                     /note="derived from carp; contains beta actin intron 1"
     primer_bind     complement(2955..2982)
                     /label=MISC-bact_intron-R1
                     /note="MISC-bact_intron-R1"
     CDS             3015..3713
                     /label=TagBFP
                     /note="monomeric blue fluorescent protein"
     misc_feature    3735..3862
                     /note="derived from carp; contains beta actin splice donor
                     from exon/intron 1 boundary"
     protein_bind    complement(3882..3915)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3949..3967
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   3972..4189
                     /label=Tol2
                     /note="Tol2"
     CDS             4254..5060
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      5206..5794
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"